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STUDIES ON YEAST DIFFERENTIATION USING ORGANIC ACID METABOLITES PART 1. DEVELOPMENT OF METHODOLOGY USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
Author(s) -
Bell D. J.,
Blake J. D.,
Prazak M.,
Rowell D.,
Wilson P. N.
Publication year - 1991
Publication title -
journal of the institute of brewing
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 51
eISSN - 2050-0416
pISSN - 0046-9750
DOI - 10.1002/j.2050-0416.1991.tb01072.x
Subject(s) - phosphoric acid , chromatography , chemistry , yeast , organic acid , fatty acid , quantitative analysis (chemistry) , biochemistry , organic chemistry
The separation of organic acids on the column Aminex HPX‐87H from Bio‐Rad is described. The relationship between column temperature and retention time was studied for both sulphuric and phosphoric acid eluants and an analytical scheme using 0.2% (v/v) phosphoric acid was developed. While the procedure is designed for the commonly occurring non‐volatile acid metabolites it was demonstrated that conditions could be established for the analysis of fatty acids to C 10 . Difficulties in the qualitative and quantitative analysis of organic acids in beer are discussed.