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A MINI‐COLUMN SCREENING TEST FOR AFLATOXINS IN SORGHUM MALT
Author(s) -
RUFFELL P. L.,
TRINDER D. W.
Publication year - 1990
Publication title -
journal of the institute of brewing
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 51
eISSN - 2050-0416
pISSN - 0046-9750
DOI - 10.1002/j.2050-0416.1990.tb01010.x
Subject(s) - aflatoxin , cartridge , sorghum , column (typography) , chromatography , clean up , detection limit , environmental science , chemistry , mathematics , food science , biology , extraction (chemistry) , engineering , agronomy , mechanical engineering , geometry , connection (principal bundle)
The development of a mini‐column screening test for aflatoxins in sorghum malt and the finally adopted method is described. The method is based on a silica cartridge clean‐up of a chloroform extract of malt followed by detection by means of an alumina/Florisil mini‐column. The method is rapid, economical, more sensitive than published methods and suitable for use by semi‐skilled personnel. It was deliberately designed to maximise test readings, thereby obviating false negative findings. Validation trials indicated that the method had a detection limit of approximately 3 μg aflatoxin B t /kg and was robust enough for routine use. The method is recommended for industrial use, provided that positive samples are confirmed by conventional analysis.