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ESTIMATION OF FERMENTABLE CARBOHYDRATES — COLLABORATIVE TRIAL
Author(s) -
Buckee G. K.,
Baker C. D.
Publication year - 1989
Publication title -
journal of the institute of brewing
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 51
eISSN - 2050-0416
pISSN - 0046-9750
DOI - 10.1002/j.2050-0416.1989.tb04617.x
Subject(s) - brewing , maltotriose , chromatography , reproducibility , maltose , repeatability , chemistry , fructose , sucrose , fermentation , food science
A procedure relying on high performance liquid chromatography, for the estimation of fermentable carbohydrates in wort, has been tested collaboratively and adopted by the Institute of Brewing Analysis Committee as a Recommended Method. A radially compressed cartridge containing silica modified with tetraethylenepentamine is used with an eluting solvent of acetonitrile/water. The results of a preliminary trial indicated that better precision is achieved using manual measurements of peak height and omitting an internal standard. In the main trial where 5 laboratories analysed 5 sample pairs the repeatability (r 95 ) and reproducibility (R 95 ) values found with their mean concentration levels (m) to which they are applicable were: fructose (0.0256 + 0.0660m) and (0.0711 + 0.0691m) for 0.17–1.01 g/100 ml; glucose 0.090m and 0.139m for 0.80–2.07 g/100 ml; sucrose 0.095 and (0.0746 + 0.1663m) for 0.12–3.88 g/100 ml; maltose 0.219 and 0.917 for 4.17–6.09 g/100 ml; maltotriose 0.122 and 0.236 for 0.97–1.19 g/100 ml. The precision values for the sums of the total fermentable sugars were 0.578 and 1.040 for 6.71–14.24 g/100 ml.