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EXTRACELLULAR PROTEASE ACTIVITY IN A STRAIN OF SACCHAROMYCES CEREVISIAE
Author(s) -
Sturley S. L.,
Young T. W.
Publication year - 1988
Publication title -
journal of the institute of brewing
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 51
eISSN - 2050-0416
pISSN - 0046-9750
DOI - 10.1002/j.2050-0416.1988.tb04549.x
Subject(s) - extracellular , proteases , protease , saccharomyces cerevisiae , intracellular , biology , autolysis (biology) , yeast , mutant , biochemistry , gene , enzyme , microbiology and biotechnology
The isolation and analysis of a strain of laboratory yeast with extracellular protease activity is described. The proteolytic activity found in culture supernatants exceeded the parental strain by at least an order of magnitude and apparently was due neither to cell lysis nor to increased cell wall permeability. The extracellular proteases were heterogeneous in composition, consisting of possibly 3 aberrantly secreted intracellular proteinases. This extracellular protease activity was conferred by a single recessive mutation ( epr1.1 ) in a gene displaying classical Mendelian inheritance. EPR1 was tentatively assigned to chromosome XV with loose linkage to the HIS3 gene. Strains carrying the mutant epr1.1 ) allele also possessed increased levels of secreted invertase activity and it is proposed that EPR1 is closely involved in the intracellular protein translocation pathway of yeast.

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