Premium
ASSAY OF MALT β‐GLUCANASE USING AZO‐BARLEY GLUCAN: AN IMPROVED PRECIPITANT
Author(s) -
McCleary Barry V.,
Shameer Ida
Publication year - 1987
Publication title -
journal of the institute of brewing
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 51
eISSN - 2050-0416
pISSN - 0046-9750
DOI - 10.1002/j.2050-0416.1987.tb04481.x
Subject(s) - glucanase , glucan , chemistry , cellulase , aqueous solution , sodium acetate , substrate (aquarium) , chromatography , nuclear chemistry , enzyme , sodium , zinc , biochemistry , organic chemistry , biology , ecology
A procedure recently described for the assay of malt β‐glucanase, which employs a dye‐labelled and chemically‐modified barley β‐glucan substrate, has been improved by changing the precipitant solution used to terminate the reaction. The new precipitant solution contains 0·4% (w/v) zinc acetate and 4% (w/v) sodium acetate dissolved in 80% (v/v) aqueous methyl cellosolve. With this precipitant the procedure can be directly applied to the assay of cellulase activity, and with minor modification, to the assay of lichenase activity.