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β‐GLUCANASE: THE SUCCESSFUL APPLICATION OF GENETIC ENGINEERING
Author(s) -
Hinchliffe Edward
Publication year - 1985
Publication title -
journal of the institute of brewing
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 51
eISSN - 2050-0416
pISSN - 0046-9750
DOI - 10.1002/j.2050-0416.1985.tb04360.x
Subject(s) - glucanase , brewing , yeast , bacillus subtilis , fermentation , enzyme , saccharomyces cerevisiae , chemistry , biochemistry , food science , biology , microbiology and biotechnology , genetics , bacteria
The now well established principles of genetic engineering are described in relation to the solution of problems associated with β‐glucans in beer. The application of these techniques has enabled the isolation of a Bacillus subtilis endo‐1, 3–1, 4‐β‐D‐glucanase gene which expresses a biologically active enzyme in yeast. 15,16 Although this enzyme is capable of hydrolysing beer β‐glucans during fermentation, thereby enhancing beer filtration, insufficient β‐glucanase is produced in yeast to enable successful commercial implementation. The requirements for the efficient production of β‐glucanase in genetically manipulated brewing yeast are described.