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RADIOIMMUNOASSAY OF PAPAIN IN BEER
Author(s) -
Rauch P.,
Fukal L.,
Strejček F.,
Káš J.
Publication year - 1984
Publication title -
journal of the institute of brewing
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 51
eISSN - 2050-0416
pISSN - 0046-9750
DOI - 10.1002/j.2050-0416.1984.tb04279.x
Subject(s) - papain , radioimmunoassay , chemistry , chromatography , incubation , enzyme , biochemistry
A radioimmunoassay (RIA) of papain is described which is capable of detecting 0·2 μg of papain/ml in beer, a level approximately 1% of that normally used for chillproofing. Changes in incubation conditions significantly accelerates the papain determination with only slight loss of accuracy. Apart from the high sensitivity it has been shown that the method is highly specific and distinguishes papain from the other chillproofing enzymes used.