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RECOGNITION OF TWO LIPASES FROM BARLEY AND GREEN MALT
Author(s) -
Baxter E. Denise
Publication year - 1984
Publication title -
journal of the institute of brewing
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 51
eISSN - 2050-0416
pISSN - 0046-9750
DOI - 10.1002/j.2050-0416.1984.tb04273.x
Subject(s) - lipase , triolein , endosperm , germination , oleic acid , chemistry , enzyme , food science , molecular mass , triacylglycerol lipase , biochemistry , biology , chromatography , botany
Lipase was extracted from ground samples of germinating and ungerminated barley, using the detergent Triton X‐100. Enzyme activity, measured by the release of oleic acid from radioactively labelled triolein, was approximately half that in suspensions of barley flour. Two distinct lipases, both active at neutral pHs, and having similar molecular weights (around 400,000) but differing in their ionic properties, were isolated from barley. The more abundant lipase was found mainly in the embryo, while the other was located in the endosperm. Both total and extractable lipase activity increased during germination.