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A RAPID AND SIMPLE METHOD FOR THE DETERMINATION OF STARCH AND β‐GLUCAN IN BARLEY AND MALT
Author(s) -
Ahluwalia Bhavna,
Ellis Elizabeth E.
Publication year - 1984
Publication title -
journal of the institute of brewing
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 51
eISSN - 2050-0416
pISSN - 0046-9750
DOI - 10.1002/j.2050-0416.1984.tb04267.x
Subject(s) - starch , hydrolysis , glucan , chemistry , chromatography , sucrose , amylase , food science , extraction (chemistry) , polysaccharide , acid hydrolysis , biochemistry , enzyme
A simple and precise method suitable for the routine determination of starch and β‐glucan in barley and malt is described. Perchloric acid (50 mM) was used to effect rapid (3 min) and exhaustive extraction of both glucans which were then measured directly from this single extract by specific enzymic hydrolysis of the individual glucans to glucose. The glucose was also measured enzymically. Little or no acid hydrolysis of starch or β‐glucan was observed under the extraction conditions used; most or all of the free glucose could be attributed to hydrolysis of sucrose. Complete solubilisation of the gum and hemicellulosic components of β‐glucan was achieved. Preincubation of the acid extracts with protease prior to amyloglucosidase digestion resulted in higher measurements (approximately 4% w/w) of starch. The method was used to measure the levels of starch and β‐glucan in five varieties of barley with contrasting malting quality, in micro‐malts prepared from these samples and in commercial lager and ale malts.