Premium
A SIMPLE AND RAPID METHOD FOR DETERMINATION OF METAL IONS IN DEVELOPING BARLEY SEEDS
Author(s) -
Ahluwalia Bhavna,
Duffus Carol M.
Publication year - 1983
Publication title -
journal of the institute of brewing
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 51
eISSN - 2050-0416
pISSN - 0046-9750
DOI - 10.1002/j.2050-0416.1983.tb04138.x
Subject(s) - simple (philosophy) , metal ions in aqueous solution , chemistry , metal , ion , chromatography , computer science , organic chemistry , philosophy , epistemology
A simple, rapid and accurate procedure involving flame—and flameless—atomic absorption spectroscopy was devised for the extraction and assay of major and trace metal ions in developing embryos and endosperms of barley seeds. Both embryo (maximum dry weight 20 mg) and endosperm (maximum dry weight 300 mg) samples were decomposed by digestion with nitric acid to yield a clear solution from which all the elements were analysed. Potassium, magnesium, calcium, iron, manganese and copper were determined from the embryo digests. Similarly, potassium, magnesium, calcium and manganese were determined from the endosperm digests. The validity of the procedure was confirmed by high recoveries of added metal ions. The sensitivity of analysis was such that sample size was in the range 1–12 mg.