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PRECIPITATION TESTS AS AN AID TO THE CLASSIFICATION OF PEDIOCOCCI AND AEROCOCCI
Author(s) -
Clausen O. G.,
Hegna Ida K.,
Kjeldaas Liv,
Palmer Lise,
Tjade O. H.
Publication year - 1981
Publication title -
journal of the institute of brewing
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 51
eISSN - 2050-0416
pISSN - 0046-9750
DOI - 10.1002/j.2050-0416.1981.tb04003.x
Subject(s) - antiserum , pediococcus , heterologous , antigen , microbiology and biotechnology , biology , chemistry , bacteria , biochemistry , immunology , lactobacillus , genetics , gene
The possibility of using simple precipitation reactions for pediococci and aerococci, viz. the standard ‘ring test’ performed in microcontainers and the gel‐diffusion test method, has been examined. With both methods, antigen extracts of the pediococci (13 strains) precipitated 100% with both homologous and heterologous pediococcus antisera and only approx. 15% with aerococcus antisera. Antigen extracts of the aerococci (12 strains) precipitated with homologous antisera at the rate of approx. 91% (ring test) and 82% (gel‐diffusion test), whereas the positive reaction with heterologous aerococcus antisera was at the rate of only 10.9% (ring test) and 5.4% (gel‐diffusion test). No precipitation was observed between antigen extracts of the aerococci and antisera of the pediococci, and only a few reactions between aerococcus antisera and pediococcus antigens were registered. The results indicate that the pediococci tested represent a serological homogenous bacterial group, whereas the aerococci lack such homogenity.