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DETERMINATION OF DNA AND OF OTHER NUCLEIC ACID FRACTIONS IN SACCHAROMYCES CEREVISIAE
Author(s) -
Trevelyan William E.
Publication year - 1980
Publication title -
journal of the institute of brewing
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 51
eISSN - 2050-0416
pISSN - 0046-9750
DOI - 10.1002/j.2050-0416.1980.tb06857.x
Subject(s) - guanine , nucleic acid , biochemistry , dna , yeast , saccharomyces cerevisiae , trichloroacetic acid , rna , nucleotide , chemistry , perchloric acid , purine , purine metabolism , nucleic acid metabolism , chromatography , enzyme , organic chemistry , gene
After extraction overnight of Saccharomyces cerevisiae (baker's yeast) with dilute alkali, to remove nucleotide pools and RNA, acidification gave an insoluble residue from which DNA guanine and adenine were extracted, in the free form, by 1M perchloric acid at room temperature, and determined by cation‐exchange chromatography. Percentage DNA in the yeast was computed as 0.062 × μmol/g of guanine + adenine. Preliminary extraction of nucleotides was unnecessary, and gave low results if acid reagents (perchloric or trichloroacetic acids) were used. Several reagents were tested for their ability to extract the nucleotide pool from intact yeast without significant effect on cell RNA content. The content of guanine + adenine in pool‐free yeast was taken as giving the sum of RNA + DNA purines, RNA purines being found by difference.