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THE DETERMINATION OF BARLEY α‐AMYLASE ACTIVITY
Author(s) -
Smith M. T.,
CornishBowden A.,
Briggs D. E.
Publication year - 1979
Publication title -
journal of the institute of brewing
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 51
eISSN - 2050-0416
pISSN - 0046-9750
DOI - 10.1002/j.2050-0416.1979.tb06847.x
Subject(s) - amylase , graph , starch , calculator , iodine , chemistry , computer science , enzyme , mathematics , chromatography , biochemistry , organic chemistry , theoretical computer science , operating system
Starch‐iodine colour (S.I.C.) units of α‐amylase activity are re‐defined. Two improved, alternative methods of calculating results are given, either (a) by a revised graphical procedure used in conjunction with a standard graph, or (b) by a graphical procedure using points calculated from the experimental results with an empirical equation using a programmed electronic calculator. Semiautomation of the measurement of starch‐iodine colours usefully reduces the work load involved in this enzyme assay. Enzyme activities may be expressed in S.I.C. units, or as a rate of increase in reducing power.