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CELL‐FREE SYNTHESIS OF ETHYL ACETATE BY EXTRACTS FROM SACCHAROMYCES CEREVISIAE
Author(s) -
Howard Deborah,
Anderson R. G.
Publication year - 1976
Publication title -
journal of the institute of brewing
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 51
eISSN - 2050-0416
pISSN - 0046-9750
DOI - 10.1002/j.2050-0416.1976.tb03728.x
Subject(s) - ethyl acetate , saccharomyces cerevisiae , ethanol , chemistry , enzyme , fractionation , biochemistry , magnesium , magnesium acetate , cell free system , chromatography , yeast , organic chemistry
A cell‐free preparation has been obtained from Saccharomyces cerevisiae which will synthesize ethyl acetate from ethanol and acetyl‐CoA. Ethyl acetate synthesizing activity is stimulated by magnesium ions, the optimal concentration being 8 mM. Subcellular fractionation indicates that the enzyme is associated with material sedimenting at 105 000 g .