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PARTITION CHROMATOGRAPHY OF SUGARS IN WORT AND BEER ON ANION EXCHANGE RESINS
Author(s) -
Havlicek J.,
Samuelson O.
Publication year - 1975
Publication title -
journal of the institute of brewing
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 51
eISSN - 2050-0416
pISSN - 0046-9750
DOI - 10.1002/j.2050-0416.1975.tb03708.x
Subject(s) - chemistry , monosaccharide , elution , orcinol , chromatography , partition coefficient , ion exchange , ethanol , ion chromatography , ion exchange resin , periodate , organic chemistry , ion
Partition chromatography on anion exchange resins in the sulphate form in 85% ethanol at 75°C was used for the separation of monosaccharides in beer and wort. The eluate was analysed automatically by the orcinol method. Simultaneous analysis by a periodate‐formaldehyde method permitted determination of glycerol. Oligomers, except for those precipitated by 65% ethanol, were separated and characterized by chromatography in this medium. Sharp separations of disaccharides were made at an intermediate concentration. Species which were not separated on this resin were resolved on a cation exchanger in its lithium form. The identification of oligomers was facilitated by the fact that for oligomers having the same type of glycosidic bonds there exists a straight line relationship between the logarithm of the distribution coefficient and the number of monosaccharide residues.