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FLOCCULATION—SOME OBSERVATIONS ON THE SURFACE CHARGES OF YEAST CELLS
Author(s) -
Fisher D. J.
Publication year - 1975
Publication title -
journal of the institute of brewing
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 51
eISSN - 2050-0416
pISSN - 0046-9750
DOI - 10.1002/j.2050-0416.1975.tb03670.x
Subject(s) - yeast , saccharomyces cerevisiae , flocculation , calcium , surface charge , biochemistry , strain (injury) , chemistry , growth medium , stationary phase , biophysics , electrophoresis , cell , chromatography , biology , bacteria , genetics , organic chemistry , anatomy
Surface charges on flocculent and non‐flocculent yeast cells have been measured by micro‐electrophoresis. Yeasts were grown both in calcium deficient and in complete medium and particular attention was paid to changes as cells passed from a logarithimic to a stationary phase of growth. Many of the ionogenic groups contributing to surface charge are situated well within the cell wall; charges on cells from the calcium‐deficient medium were higher than on cells from the complete medium. A pH‐dependent rearrangement of an underlying surface protein layer is postulated for the flocculent yeast Saccharomyces cerevisiae Strain NCYC 1109. No evidence of a similar rearrangement was found with other flocculent strains examined. The results are discussed in relation to ‘calcium bridge’ formation.