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IDENTIFICATION OF ENZYMES AS GLYCOPROTEINS CONTAINING GLUCOSE OR MANNOSE
Author(s) -
BøgHansen T. C.,
Brogren C.H.,
McMurrough I.
Publication year - 1974
Publication title -
journal of the institute of brewing
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 51
eISSN - 2050-0416
pISSN - 0046-9750
DOI - 10.1002/j.2050-0416.1974.tb06793.x
Subject(s) - concanavalin a , mannose , biochemistry , esterase , enzyme , glycoprotein , carbohydrate , chemistry , affinity electrophoresis , acid phosphatase , affinity chromatography , in vitro
One‐dimensional affinity electrophoresis and crossed immuno‐affinoelectrophoresis with concanavalin A have been applied to show that esterase and acid phosphatase from barley malt are glycoprotein enzymes containing glucose or mannose in their carbohydrate part. The enzymes retained their enzymic activity after binding in macromolecular complexes with concanavalin A. Indications were found of micro‐heterogeneity within the carbohydrate moiety of the esterase.