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NUCLEOTIDES IN BEER
Author(s) -
Steward S. R.,
Smith J. L.,
Kavanagh T. E.,
Hildebrand R. P.,
Clarke B. J.
Publication year - 1974
Publication title -
journal of the institute of brewing
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 51
eISSN - 2050-0416
pISSN - 0046-9750
DOI - 10.1002/j.2050-0416.1974.tb03580.x
Subject(s) - chemistry , chromatography , guanosine , column chromatography , sephadex , fraction (chemistry) , ion chromatography , nucleotide , yield (engineering) , biochemistry , enzyme , gene , materials science , metallurgy
Nucleotidic material was isolated by anion exchange chromatography of beer on a Dowex 1 × 8 (formate) column. Ultraviolet spectroscopy and retention volume data suggested the presence of cytidine monophosphate (CMP) [max. 5 mg/litre], in one of the fractions. No other mononucleotides were detected. Chromatography of charcoal extracts of beer on DEAE Sephadex A25 failed to yield a fraction containing mononucleotides. Triangular taste tests indicated that addition of adenosine‐5′‐monophosphate (AMP) and guanosine‐5′‐monophosphate (GMP) to beer (30 mg/litre and 25 mg/litre respectively) did not cause significant flavour changes.