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STUDIES ON THE FORMATION OF SPHEROPLASTS FROM STATIONARY PHASE CELLS OF SACCHAROMYCES CEREVISIAE
Author(s) -
Russell Inge,
Garrison Ivan F.,
Stewart Graham G.
Publication year - 1973
Publication title -
journal of the institute of brewing
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 51
eISSN - 2050-0416
pISSN - 0046-9750
DOI - 10.1002/j.2050-0416.1973.tb03499.x
Subject(s) - spheroplast , saccharomyces cerevisiae , protease , yeast , biochemistry , enzyme , cell wall , mannan , chemistry , stationary phase , hydrolysis , biology , biophysics , chromatography , polysaccharide , escherichia coli , gene
The rate of spheroplast formation in stationary phase cells of Saccharomyces cerevisiae has been found to be increased as a result of pre‐treatment of the cells with protease. The protease has been found selectively to remove the cell wall's mannan‐phosphate‐protein layer and thus greatly facilitate the action of snail gut enzymes or other such enzymes, in their hydrolytic activity upon the glucan of the cell wall.