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STUDIES ON CARBOHYDRATE‐METABOLIZING ENZYMES Part XXV. THE DEBRANCHING ENZYME SYSTEM IN GERMINATED BARLEY
Author(s) -
Manners D. J.,
Rowe Karen L.
Publication year - 1971
Publication title -
journal of the institute of brewing
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 51
eISSN - 2050-0416
pISSN - 0046-9750
DOI - 10.1002/j.2050-0416.1971.tb03385.x
Subject(s) - amylopectin , chemistry , glycogen debranching enzyme , biochemistry , enzyme , chromatography , size exclusion chromatography , starch , glycogen synthase , amylose
Extracts of germinated barley contain two debranching enzymes which have been separated from each other, and from α‐amylase, by continuous electrophoresis followed by gel filtration. Amylopectin 6‐glucanohydrolase hydrolyses the outer‐most inter‐chain linkages in amylopectin but has no action on glycogen or on α‐limit dextrins. Limit dextrinase has no action on amylopectin, but readily hydrolyses α‐limit dextrins; it differs fron amylopectin 6‐glucanohydrolase in having a relatively lower activity at pH 4, pH 7 or 60° C, and is selectively activated by chloride ions.