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FLOCCULATION OF BREWER'S YEAST
Author(s) -
Lyons T. P.,
Hough J. S.
Publication year - 1970
Publication title -
journal of the institute of brewing
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 51
eISSN - 2050-0416
pISSN - 0046-9750
DOI - 10.1002/j.2050-0416.1970.tb03343.x
Subject(s) - flocculation , cell wall , phosphodiester bond , fractionation , phosphate , phosphorus , fraction (chemistry) , saccharomyces cerevisiae , yeast , ethylenediamine , lysis , chemistry , calcium , chromatography , biochemistry , biology , organic chemistry , rna , gene
Analyses of cell walls isolated from flocculent and non‐flocculent strains of Saccharomyces cerevisiae revealed no appreciable differences in the levels of major components. Fractionation using ethylenediamine according to the method of Korn & Northcote furnished a Fraction A from flocculent walls which had higher levels of phosphorus than that from non‐flocculent. 70% of the phosphorus of this fraction was incorporated as phosphodiester in a manno‐protein. Walls from flocculent yeasts bound on average twice as much calcium as did walls from non‐flocculent yeasts. Removal of the phosphorylated manno‐protein decreased the capacity of the cell wall to bind calcium and rendered the wall non‐flocculent, emphasizing the role of superficial phosphate groups in flocculation.