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INCORPORATION OF U‐ 14 C‐ AND 15 N‐LEUCINE BY EXCISED BARLEY EMBRYOS
Author(s) -
Pragnell M. J.,
Jones Margaret,
Pierce J. S.,
Mikola J.,
Enari TM.
Publication year - 1969
Publication title -
journal of the institute of brewing
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 51
eISSN - 2050-0416
pISSN - 0046-9750
DOI - 10.1002/j.2050-0416.1969.tb03240.x
Subject(s) - transamination , labelling , leucine , amino acid , embryo , hydrolysis , chemistry , biochemistry , biology , genetics
Sterile excised barley embryos were allowed to germinate in a medium containing U‐ 14 C‐ and 15 N‐ l ‐leucine. After periods of 3 and 5 days, the whole embryos were subjected to acid hydrolysis and the resulting amino acids were analysed for isotopic labelling. While there was no significant spread of the 14 C labelling to other amino acids, the appearance of 15 N in all the amino acids showed that extensive transamination had occurred.