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A RAPID METHOD FOR DETERMINING BIOTIN ACTIVITY IN RAW MATERIALS FOR FERMENTATION
Author(s) -
Danielsen Svend,
Eriksen Bjorn W.
Publication year - 1968
Publication title -
journal of the institute of brewing
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 51
eISSN - 2050-0416
pISSN - 0046-9750
DOI - 10.1002/j.2050-0416.1968.tb03169.x
Subject(s) - biotin , yeast , biocytin , fermentation , agar plate , raw material , agar , serial dilution , biochemistry , chemistry , incubation , chromatography , biology , bacteria , organic chemistry , medicine , genetics , alternative medicine , pathology , neuroscience , central nervous system
The biotin activity of a raw material towards yeast may consist of the sum of the activities of biotin, desthiobiotin, oxybiotin, biocytin and possibly other factors. Some of these substances only stimulate certain strains of yeast; on the other hand, the strains of lactobacilli generally used for estimation of biotin activity utilize only D‐biotin. It is thus desirable to have a method available in which a manufacturer's own strain of yeast is used as test organism for biotin activity. A simple method for biotin determination based on the cup plate assay is described. Biotin‐free agar is inoculated with yeast and a series of standard solutions of D‐biotin and a series of dilutions of the raw material under investigation are placed in holes cut in the agar. After incubation, the zones of growth are measured and from their diameters the biotin activity can be calculated.