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STUDIES ON THE PROTEOLYTIC ENZYMES OF A COMMERCIAL MALT
Author(s) -
Bhatty R. S.
Publication year - 1968
Publication title -
journal of the institute of brewing
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 51
eISSN - 2050-0416
pISSN - 0046-9750
DOI - 10.1002/j.2050-0416.1968.tb03143.x
Subject(s) - bromate , chemistry , proteolytic enzymes , enzyme , thiol , trypsin , biochemistry , chromatography , organic chemistry , ion
Two proteolytic enzymes, Hb‐ase and BAPA‐ase, were partially purified from commercial malt. Hb‐ase was inhibited by bromate, N‐ethylmaleimide and p‐chloromercuriphenylsulphonic acid and activated by ethylenediaminetetra‐acetic acid. BAPA‐ase was inhibited by N‐ethylmaleimide and p‐hydroxychloromercuribenzoate but not by bromate or phenylmethylsulphonylfluoride, a specific trypsin inhibitor. The results obtained suggested that BAPA‐ase, like the Hb‐ase, is a thiol enzyme.