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QUANTITATIVE GAS CHROMATOGRAPHIC ANALYSIS OF AMINO ACIDS IN BEER AND WORT
Author(s) -
Kurosky Alexander,
Bars Austra
Publication year - 1968
Publication title -
journal of the institute of brewing
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 51
eISSN - 2050-0416
pISSN - 0046-9750
DOI - 10.1002/j.2050-0416.1968.tb03116.x
Subject(s) - valine , chemistry , amino acid , phenylalanine , methionine , alanine , leucine , isoleucine , chromatography , glycine , threonine , aspartic acid , proline , amino acid synthesis , organic chemistry , serine , lysine , biochemistry , enzyme
Amino acids present in beer and wort samples were isolated using Dowex 50W‐X8 ion exchange resin. These acids were converted to N‐trifluoroacetyl methyl esters and were separated on a 10 ft. x 1/4 in. copper column packed with 3/4% Carbowax 1540 and 1/4% NPGS on Chromosorb W. Complete separation and quantitative estimation was obtained for eleven amino acids: valine, alanine, isoleucine, leucine, glycine, proline, aspartic acid, threonine, methionine, glutamic acid, and phenylalanine. Standards of the acids showed a relative standard deviation of 4% and less for nine acids while threonine and methionine gave deviations of 9% and 15% respectively. The other naturally‐occurring amino acids did not interfere with the separation of the above eleven. Amino acid assimilation data is presented along with other beer and wort analyses.