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THE SEPARATION OF BARLEY PROTEINS BY DISC ELECTROPHORESIS USING POLYACRYLAMIDE
Author(s) -
Brown D.,
Boyd W. J. R.
Publication year - 1966
Publication title -
journal of the institute of brewing
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 51
eISSN - 2050-0416
pISSN - 0046-9750
DOI - 10.1002/j.2050-0416.1966.tb03002.x
Subject(s) - polyacrylamide gel electrophoresis , chromatography , electrophoresis , chemistry , polyacrylamide , separation (statistics) , biochemistry , computer science , enzyme , polymer chemistry , machine learning
Protein components extracted from the barley varieties, Prior and Beecher, have been electrophoretically separated in acrylamide gels. The resolution obtained was sufficiently definitive to allow scanning by transmission densitometry. With the same solvent, similar electrophoretic patterns were obtained over a number of sequential extractions. However, with different solvents the patterns showed that individual proteins had been extracted in different proportions. The work presented emphasizes the need for clarification of the relative importance of the separated proteins.