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YEAST GROWTH METHOD FOR ASSAY OF BIOTIN ACTIVITY
Author(s) -
Suomalainen Heikki,
Keränen A. J. A.,
Kangasperko Jaakko
Publication year - 1965
Publication title -
journal of the institute of brewing
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 51
eISSN - 2050-0416
pISSN - 0046-9750
DOI - 10.1002/j.2050-0416.1965.tb02081.x
Subject(s) - biotin , yeast , chemistry , food science , biochemistry , chromatography
Routine assays of biotin, performed according to the yeast growth method of White & Munns, sometimes gave irregular results, i.e., an exceptionally vigorous growth of the yeast in biotin‐free medium. This gave rise to uncertainty as regards the functioning of the method and the reliability of the results. The number of anomalous cases increased when the industrial stages A 2 and A 5 of baker's yeast were used as seed yeast instead of the pure culture stage R 3 . Cultures which had been contaminated with the wild yeast Pichia orientalis , isolated from commercial baker's yeast, or with a Candida utilis collection strain, revealed that the anomaly was due to an infection of the seed yeast. In the case of a slight infection, the wild yeast, not needing biotin, increases vigorously in the biotin‐free medium, although in a biotin‐containing medium baker's yeast is able to compete successfully.