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A MODIFICATION OF THE SANDSTEDT, KNEEN AND BLISH ASSAY OF α‐AMYLASE
Author(s) -
Briggs D. E.
Publication year - 1961
Publication title -
journal of the institute of brewing
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 51
eISSN - 2050-0416
pISSN - 0046-9750
DOI - 10.1002/j.2050-0416.1961.tb01819.x
Subject(s) - amylase , colorimeter , starch , chemistry , chromatography , enzyme , incubation , biochemistry , food science , physics , quantum mechanics
The Sandstedt, Kneen & Blish method for the assay of α‐amylase uses as a measure of enzyme activity the reciprocal of the time taken for a standard decrease to occur in the starch‐iodine blue colour of an incubation mixture containing a large quantity of added β‐amylase. This method has been modified so that a photoelectric colorimeter is employed in conjunction with a standard graph. The precision of the method is thereby Improved and the work needed for each assay reduced. The principles involved could easily be applied to other enzyme assays.