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THE CONCENTRATION OF MALTASE
Author(s) -
Phillips A. W.
Publication year - 1957
Publication title -
journal of the institute of brewing
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 51
eISSN - 2050-0416
pISSN - 0046-9750
DOI - 10.1002/j.2050-0416.1957.tb02921.x
Subject(s) - maltase , chemistry , food science , biochemistry , enzyme
Maltase produced by a brewing strain of Screen, cerevisiae has been purified by fractional adsorption on calcium phosphate gel followed by chromatography on alumina at constant pH by means of a concentration gradient of buffer solution. In this way, fractions have been obtained which, on the basis of their optical densities at 280 μ., are estimated to be 200 times as active as the crude starting material.