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6. COMPARATIVE SEROLOGICAL INVESTIGATIONS
Author(s) -
Fairbrothers D. E.
Publication year - 1980
Publication title -
taxon
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 81
eISSN - 1996-8175
pISSN - 0040-0262
DOI - 10.1002/j.1996-8175.1980.tb00654.x
Subject(s) - immunoelectrophoresis , biology , serology , precipitin , immunodiffusion , antiserum , ouchterlony double immunodiffusion , antigen , immunology , antibody
Summary Protein extracts from Aralidium and 23 species belonging to the families Araliaceae, Cornaceae, Garryaceae, Griseliniaceae, Nyssaceae, Saxifragaceae, and Umbelliferae were used as antigens to test with Aralidium antisera in Ouchterlony, Boyden, immunoelectrophoresis, and rocket immunoelectrophoresis (RIE) experiments. Rocket immunoelectrophoresis, a recently developed and sensitive serological technique, was the only technique which produced precipitins (rockets) in cross‐reacting experiments with any species, and then only those belonging to the Cornaceae and Nyssaceae. Therefore, based on antigenic protein information Aralidium should be treated as a monotypic genus in the family Aralidiaceae which has distant relationship, first with the Cornaceae and secondly, even more distant relationship, with the Nyssaceae. No protein similarity was detected between Aralidium and any of the other species tested. RIE proved to be a very valuable technique for use in plant systematic serological research.