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Progesterone Metabolism in Cultured Amniotic Fluid Cells
Author(s) -
Beling Carl G.,
Cederqvist Lars L.
Publication year - 1978
Publication title -
international journal of gynecology and obstetrics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.895
H-Index - 97
eISSN - 1879-3479
pISSN - 0020-7292
DOI - 10.1002/j.1879-3479.1977.tb00702.x
Subject(s) - pregnenolone , incubation , amniotic fluid , dehydrogenase , endocrinology , metabolism , medicine , cell culture , steroid , andrology , enzyme , biology , biochemistry , fetus , pregnancy , hormone , genetics
Amniotic fluid cells obtained by amniocentesis at 16–20 weeks' gestation were grown in culture until a confluent monolayer of cell had been formed. Radiolabeled pregnenolone, progesterone and 20α‐dihydroprogesterone were added to the cell cultures; steroid metabolites which formed after 24 and 48 hours of incubation were identified. Incubation of the cell cultures with pregnenolone‐ 3 H resulted in the formation of progesterone, 17α‐progesterone and 20α‐dihydroprogesterone. A significant amount of progesterone was identified after incubating the cell cultures with 20α‐dihydroprogesterone. The results indicate that 3β‐ol‐dehydrogenase, 17α‐hydroxylase and 20α‐hydroxysteroid dehydrogenase enzymes are present in cultured amniotic fluid cells obtained at 16–20 weeks' gestation.