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Detection of Tumor‐Specific Antigens in Human Mucinous Cystadenocarcinoma of the Ovary by Immunodiffusion
Author(s) -
Chow SongNan,
Chiang WenTsuo,
Wei PingYen
Publication year - 1976
Publication title -
international journal of gynecology and obstetrics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.895
H-Index - 97
eISSN - 1879-3479
pISSN - 0020-7292
DOI - 10.1002/j.1879-3479.1976.tb00611.x
Subject(s) - mucinous cystadenocarcinoma , serous cystadenocarcinoma , ouchterlony double immunodiffusion , ovary , medicine , cystadenocarcinoma , pathology , dysgerminoma , ovarian tumor , ovarian cancer , antigen , antiserum , cancer , immunology
Rabbit antiserum to a tissue extract of human mucinous cystadenocarcinoma of the ovary reacted with tissue extracts of normal ovary and various ovarian malignancies, and ascitic or cystic fluids of ovarian origin by Ouchterlony double gel diffusion and precipitin inhibition techniques. The tumor‐associated antigen(s) of mucinous cystadenocarcinoma, which were demonstrated by Ouchterlony double diffusion, were not present in tissue extract of pooled normal ovaries and cystic fluid of benign tubo‐ovarian cyst. An organ‐associated tumor antigen as well as the type‐specific tumor antigen may exist in mucinous cystadenocarcinoma of the ovary. The mucinous cystadenocarcinoma was not very immunologically different but was distinguishable from serous cystadenocarcinoma and other types of ovarian cancer by double gel diffusion. Precipitin‐inhibition reactions demonstrated that the adsorbed antiserum to human ovarian mucinous cystadenocarcinoma mixed with tissue extracts of dysgerminoma and serous cystadenocarcinoma, and ascitic fluid of papillary embryonal adenocarcinoma of the ovary could not eliminate the specific precipitin line developed with tissue extract of mucinous cystadenocarcinoma.