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Prevention by Pentoxifylline of Transient Ca 2+ Accumulation and Transglutaminase Activation in Rat Erythrocytes
Author(s) -
Swislocki Norbert I.,
Tierney Joan M.
Publication year - 1991
Publication title -
the journal of clinical pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.92
H-Index - 116
eISSN - 1552-4604
pISSN - 0091-2700
DOI - 10.1002/j.1552-4604.1991.tb03733.x
Subject(s) - tissue transglutaminase , pentoxifylline , chemistry , pharmacology , transient (computer programming) , biophysics , medicine , biochemistry , enzyme , biology , computer science , operating system
The authors have examined the effects of pentoxifylline, a drug used to improve peripheral blood flow in patients with vascular disorders, on shear‐induced periodic Ca 2+ entry and its consequences in the rat erythrocyte. To study the effects of periodic Ca 2+ entry on Ca 2+ dependent processes, erythrocytes, with and without pentoxifylline, were subjected to rotational shear produced by swirling‐cell suspensions in an isosmotic medium for 5‐second intervals. Pulses of increasing duration from 5–30 seconds promoted increased accumulation of 45 Ca 2+ ; intermittent 5 sec pulses, at 10‐minute intervals, produced a stepwise accumulation of 45 Ca 2+ . Ca 2+ accumulation was accompanied by elevated Ca 2+ dependent transglutaminase. Shear induced Ca 2+ entry and the increase in transglutaminase activity was reduced in cells treated with pentoxifylline. These findings suggest that pentoxifylline can reduce shear induced periodic Ca 2+ entry that leads to transient activation of Ca 2+ dependent transglutaminase, accumulation of crosslinked proteins, and loss of erythrocyte deformability.