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Biotransformation and Excretion: In Vivo Studies Utilizing Stable Isotopes and Nuclear Magnetic Resonance
Author(s) -
Unkefer Clifford J.
Publication year - 1986
Publication title -
the journal of clinical pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.92
H-Index - 116
eISSN - 1552-4604
pISSN - 0091-2700
DOI - 10.1002/j.1552-4604.1986.tb03557.x
Subject(s) - in vivo , nuclear magnetic resonance spectroscopy , nuclear magnetic resonance , biotransformation , isotope , chemistry , magnetic resonance imaging , metabolism , biochemistry , biology , medicine , enzyme , physics , nuclear physics , microbiology and biotechnology , radiology
Specific 13 C labeling in combination with nuclear magnetic resonance (NMR) spectroscopy is a powerful tool for the study of metabolism. Nuclear magnetic resonance allows both the extent and distribution of 13 C labels in metabolites to be quantified without the need to isolate or degrade them. In addition, NMR is nonperturbing to living cells, allowing real‐time observation of metabolic processes. To date, the application of 13 C‐NMR to in vivo studies has been confined to the study of metabolic processes in cell suspensions, perfused organs, and small animals. The continuing development of large‐bore high field magnets and surface coils will, in principle, allow in vivo metabolic studies to be extended to humans.