Premium
Determination of Adrenal Response After Oral Administration of Multiple Doses of Methylprednisolone
Author(s) -
GARG DYAL C.,
WAGNER JOHN G.,
AYRES JAMES W.,
ALBERT KENNETH S.
Publication year - 1979
Publication title -
the journal of clinical pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.92
H-Index - 116
eISSN - 1552-4604
pISSN - 0091-2700
DOI - 10.1002/j.1552-4604.1979.tb01627.x
Subject(s) - miami , pharmacy , clinical pharmacology , medicine , pharmacology , family medicine , environmental science , soil science
ORMAL cortisol production by the adrenals has a circadian rhythm, and many studies1-6 have shown a variation of plasma cortisol with the time of day. Migeon et al.7 showed that the diurnal variation of plasma levels of 17-hydroxycorticosteroids in night workers and blind persons was the same as that in normal subjects. Hellman et al.8 showed that cortisol was secreted intermittently rather than continuously. Fluorescence,9 competitive protein binding,’#{176}radioimmunoassay,11 and enzymelabeled immunoassay12 have all been used to measure plasma levels of cortisol. Highpressure liquid chromatographic (HPLC) analysis has obtained considerable attention recently for the determination of steroids in biologic fluids and pharmaceutical products.’3-16 In our laboratory, an HPLC assay was developed for simultaneous determination of both hydrocortisone and methyiprednisolone in plasma using