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Laser scanning cytometry for enumeration of fluorescent microspheres
Author(s) -
Ladner David A.,
Lee Benjamin W.,
Clark Mark M.
Publication year - 2007
Publication title -
journal ‐ american water works association
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.466
H-Index - 74
eISSN - 1551-8833
pISSN - 0003-150X
DOI - 10.1002/j.1551-8833.2007.tb07893.x
Subject(s) - enumeration , microsphere , fluorescence , cryptosporidium , cytometry , flow cytometry , fluorescence microscope , laser scanning , microscopy , materials science , cell counting , confocal laser scanning microscopy , laser , nanotechnology , biomedical engineering , chemistry , optics , biology , microbiology and biotechnology , physics , mathematics , chemical engineering , engineering , cell , biochemistry , feces , cell cycle , combinatorics
Water research has been enhanced in recent years through the use of polymer fluorescent microspheres. Microspheres have been used to simulate bacteria, viruses, Giardia cysts, and Cryptosporidium oocysts in an array of water treatment applications. Microspheres most commonly are enumerated by direct count with fluorescence microscopy, which requires skilled operators and expensive equipment. Although other methods exist, they are less accurate. A new microsphere enumeration method has been developed with laser scanning cytometry technology, and a prototype laser scanning cytometry device has been designed, built, and tested in the laboratory. The prototype performance compared well with fluorescence microscopy in counting 2‐μm microspheres. It is envisioned that a laser scanning cytometry device such as the prototype described here could be used in many applications as a fast, userfriendly, and inexpensive way to enumerate microspheres.