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Susceptibility of five strains of Cryptosporidium parvum oocysts to UV light
Author(s) -
Clancy Jennifer L.,
Marshall Marilyn M.,
Hargy Thomas M.,
Korich Dick G.
Publication year - 2004
Publication title -
journal ‐ american water works association
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.466
H-Index - 74
eISSN - 1551-8833
pISSN - 0003-150X
DOI - 10.1002/j.1551-8833.2004.tb10576.x
Subject(s) - cryptosporidium parvum , infectivity , microbiology and biotechnology , strain (injury) , inoculation , biology , ultraviolet light , staining , eosin , ultraviolet , virology , chemistry , photochemistry , materials science , virus , optoelectronics , immunology , genetics , anatomy
Previous evaluations of the effect of ultraviolet (UV) light on Cryptosporidium parvum oocysts have been limited to a single strain—the Iowa strain. This study investigated the response of five strains of C. parvum to UV. A collimated beam apparatus was used to apply controlled doses of monochromatic (254 nm) UV to oocysts of the Iowa, Moredun, Texas A&M, Maine, and Glasgow strains. Irradiation was measured using a calibrated radiometer and sensor. Inactivation was quantified through animal infectivity by inoculation of cohorts of CD‐1 neonatal mice with UV‐treated and untreated control oocysts of each strain followed by examination of intestinal sections for infection using hemotoxylin and eosin staining. A UV light dose of 10 mJ/cm 2 achieved at least 4‐log 10 inactivation of all strains evaluated. All five strains of C. parvum were shown to be highly susceptible to low levels of UV light.