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Evaluation of four commercial antibodies
Author(s) -
Hoffman Rebecca,
Chauret Christian,
Standridge Jon,
Peterson Linda
Publication year - 1999
Publication title -
journal ‐ american water works association
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.466
H-Index - 74
eISSN - 1551-8833
pISSN - 0003-150X
DOI - 10.1002/j.1551-8833.1999.tb08698.x
Subject(s) - avidity , antibody , cryptosporidium , giardia , cryptosporidium parvum , biology , cross reactivity , virology , microbiology and biotechnology , immunology , cross reactions , feces
Evaluation of commercially available anti‐Cryptosporidium and anti‐Giardia antibodies indicates each offers advantages and disadvantages. Flow cytometer‐derived fluorescence measurements were used to evaluate lot‐to‐lot variability, cross‐reactivity, and avidity of four antibodies. Variability within kit 3 and 4 antibody lots was great, whereas kits 1 and 2 stained parasites consistently. Kit 3 and 4 antibodies cross‐reacted with non‐ parvum Cryptosporidium ; kit 1 and 4 antibodies cross‐reacted with Giardia muris. Kit 1 (primary only), 2, and 3 anti‐ Cryptosporidium antibodies formed low‐avidity complexes, unlike those in kits 1 (secondary) and 4 and all Giardia antibodies. Additionally, the authors quantified the ability of antibodies to bind stressed and unstressed parasites following methodological processing. The studies identified significant differences in antibody characteristics and performance.