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Environmental methods for Cryptosporidium
Author(s) -
Jakubowski Walter,
Boutros Susan,
Faber Walter,
Fayer Ron,
Ghiorse William,
LeChevallier Mark,
Rose Joan,
Schaub Stephen,
Singh Ajaib,
Stewart Mic
Publication year - 1996
Publication title -
journal ‐ american water works association
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.466
H-Index - 74
eISSN - 1551-8833
pISSN - 0003-150X
DOI - 10.1002/j.1551-8833.1996.tb06617.x
Subject(s) - cryptosporidium , sampling (signal processing) , biochemical engineering , task force , risk analysis (engineering) , environmental science , computer science , business , biology , engineering , ecology , filter (signal processing) , public administration , political science , computer vision , feces
A number of approaches offer promise for addressing the limitations of current antibody‐based detection methods. This report was prepared by the Working Group on Waterborne Cryptosporidiosis (Technical Task Force E, Developmental Status of Environmental Sampling, Water Testing, and Surrogate Indicators). Methods for detecting Cryptosporidium oocysts in water have centered around microscopic examination of fluorescent antibody‐stained concentrates from large‐volume water samples. The limitations of these antibody‐based methods include the need for experienced analysts, lengthy analytical time, expense, lack of specificity, erratic efficiency, low precision, and difficulty in determining viability. A number of methods, assays, and procedures that have the potential for ameliorating some of these limitations are currently being evaluated. How successful such processes will be remains to be demonstrated by the scientific community.