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Investigating the Mechanism of Inactivation of Escherichia coli B by Monochloramine
Author(s) -
Jacangelo Joseph G.,
Olivieri Vincent P.,
Kawata Kazuyoshi
Publication year - 1991
Publication title -
journal ‐ american water works association
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.466
H-Index - 74
eISSN - 1551-8833
pISSN - 0003-150X
DOI - 10.1002/j.1551-8833.1991.tb07152.x
Subject(s) - escherichia coli , disinfectant , bacteria , cell envelope , chemistry , nucleic acid , microbiology and biotechnology , organism , biophysics , biochemistry , biology , paleontology , genetics , organic chemistry , gene
To better understand the mode of action of monochloramine, its effect on the structural and physiological integrity of Escherichia coli B was studied. The disinfectant did not severely damage the cell envelope of the bacterium or affect nucleic acid function. Inhibition of typical protein‐associated biological activities, e.g., bacterial transport, respiration, and substrate dehydrogenation, were observed at concentrations normally used for disinfection. Differences in rates of kill and inhibition of various biological activities suggest that the shoulder formation on inactivation curves was due to reactions at many sensitive sites in the bacterium, the extent of which determined whether the organism remained recoverable.