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New approaches to measuring total bacterial populations in water supplies
Author(s) -
Herson Diane S.,
Baker Katherine H.
Publication year - 1982
Publication title -
journal ‐ american water works association
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.466
H-Index - 74
eISSN - 1551-8833
pISSN - 0003-150X
DOI - 10.1002/j.1551-8833.1982.tb04999.x
Subject(s) - acridine orange , tetrazolium chloride , bacteria , staining , chromatography , membrane , gelatin , formazan , chemistry , filter paper , membrane filter , biochemistry , biology , medicine , genetics , ischemia , cardiology
Direct counts of stained bacterial cells were compared with counts obtained from standard plating techniques. The method used by the authors combined acridine orange staining with 2‐( p ‐iodophenyl)‐3‐( p ‐nitrophenyl)‐5‐phenyl‐tetrazolium chloride reduction. To overcome the interference of a membrane filter's pores with the identification of stained cells, the authors used a gel‐stripping technique to transfer the bacterial cells from membrane filters to a gelatin matrix before counts were made. The method will supplement standard plate counts by determining metabolically active bacteria in a water sample within four hours.