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Detecting an algal toxin by high‐pressure liquid chromatography
Author(s) -
Wong See Hoi,
Hindin Ervin
Publication year - 1982
Publication title -
journal ‐ american water works association
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.466
H-Index - 74
eISSN - 1551-8833
pISSN - 0003-150X
DOI - 10.1002/j.1551-8833.1982.tb04996.x
Subject(s) - chromatography , aqueous solution , chemistry , acetonitrile , high pressure , hexane , anabaena , ultraviolet , high performance liquid chromatography , neurotoxin , cyanobacteria , materials science , organic chemistry , biochemistry , optoelectronics , engineering physics , biology , bacteria , engineering , genetics
A high‐pressure liquid chromatographic method is proposed for isolating and quantifying aqueous solutions of anatoxin‐a, a neurotoxin produced by certain strains of Anabaena flos‐aquae. A normal phase Silica‐A column with an 80 percent solution of isopropanol in hexane or a C 18 reverse phase column with an 80 percent aqueous solution of acetonitrile gave good separation. Ultraviolet spectrophotometric detection at 227 nm provided for optimum quantification.