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Detection of Coliform Organisms by the Fluorescent‐Antibody Method
Author(s) -
Lieber Maxim,
Martin Alice Joan
Publication year - 1965
Publication title -
journal ‐ american water works association
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.466
H-Index - 74
eISSN - 1551-8833
pISSN - 0003-150X
DOI - 10.1002/j.1551-8833.1965.tb01376.x
Subject(s) - fluorescence , fluorescein , antiserum , antigen , antibody , fluorescence microscope , biology , microbiology and biotechnology , chromatography , chemistry , virology , optics , immunology , physics
This article discusses experimentation with the use of the fluorescent‐antibody (FA) technique as a test for the presence of coliform organisms in water. The method involves the use of a fluorescence microscope on unknown cultures (antigens) treated with solutions of fluorescein‐labeled antisera. The basic principle of the FA technique is presented and discussed in the article.