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Presterilized Liquid‐Liquid Transfer Loops in Water Bacteriology
Author(s) -
Connors Joseph J.
Publication year - 1963
Publication title -
journal ‐ american water works association
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.466
H-Index - 74
eISSN - 1551-8833
pISSN - 0003-150X
DOI - 10.1002/j.1551-8833.1963.tb01013.x
Subject(s) - loop (graph theory) , water transfer , tube (container) , distilled water , series (stratigraphy) , materials science , transfer (computing) , closed loop transfer function , control theory (sociology) , chemistry , composite material , transfer function , engineering , mathematics , computer science , chromatography , environmental science , electrical engineering , geology , paleontology , control (management) , combinatorics , artificial intelligence , parallel computing , water resource management
This article discusses the relative advantages of a newly designed transfer loop which tends to obviate defects inherent in the traditional wire loop. Characteristics of the new transfer loop are discussed and compared to the traditional wire loop. The article discusses a series of transfers that were made to determine the range of inoculum delivered by the loops under both conditions of use: in the first series, twenty transfers were made with a commercial, prefabricated loop, submerging only the loop end and not its shank in the broth; in the second series, twenty transfers were made with the new stainless steel loop, submerging only the loop end and not its shank in the broth; and, in the third series, twenty transfers were made with the new stainless steel loop, inserting the loop to the bottom of the broth tube, and withdrawing it with the loop end held perpendicular to, and in contact with, the side of the tube. The loop was similarly inserted to the bottom of the distilled water tube to remove the entire amount carried by the loop.