Open AccessThe Rat Corpulent ( cp ) Mutation Maps to the Same Interval on ( Pgm1‐Glut1 ) Rat Chromosome 5 as the Fatty ( fa ) Mutation
Author(s) -
Kahle E. Bowie,
Butz Kenneth G.,
Chua Streamson C.,
Kershaw Erin E.,
Leibel Rudolph L.,
Fenger Terry W.,
Hansen Carl T.,
Michaelis Otho E.
Publication year - 1997
Publication title -
obesity research
Language(s) - English
Resource type - Journals
eISSN - 1550-8528
pISSN - 1071-7323
DOI - 10.1002/j.1550-8528.1997.tb00654.x
Subject(s) - complementation , genetics , mutation , biology , gene , microbiology and biotechnology , phenotype
The autosomal recessive obesity mutations fatty ( fa ) and corpulent ( cp ) arose in separate rat strains, 13M and Koletsky, respectively. By complementation analysis, the two mutations appear to be in the same gene. The somewhat different phenotypes of fa/fa and cp/cp animals probably reflect the fact that the mutations are segregating on different rat strains. The fa mutation has been mapped to the interval between Pgm1 and Glut1 on rat Chr 5, but cp has not been mapped genetically. We mapped cp in 30 obese progeny of a LA/N‐BN cp /+ intercross using microsatellite markers for these flanking genes. Cp maps to the same genetic interval as rat fa and mouse db . Cp is flanked by Glut1 and Pgm1 : Pgm1——–cp——–Glut1 map distance (cM) 1.67 6.67 Thus, cp and fa map to the same ∼8 cM interval of the rat genome. In conjunction with the complementation studies alluded to above, these findings indicate that cp and fa are mutations in the same gene ( Lepr ).