Open AccessPositive Modulation of the Adipoconversion of Adipoblasts Derived from Genetically Obese Rats by Sodium Butyrate
Author(s) -
Gregoire Francine M.,
Magrurn; Linda,
Johnson Patricia R.,
Greenwood MRC
Publication year - 1994
Publication title -
obesity research
Language(s) - English
Resource type - Journals
eISSN - 1550-8528
pISSN - 1071-7323
DOI - 10.1002/j.1550-8528.1994.tb00636.x
Subject(s) - sodium butyrate , endocrinology , medicine , butyrate , adipose tissue , population , obesity , chemistry , biology , biochemistry , gene , environmental health , fermentation
Using a new serum‐free primary culture system, we have previously reported genotypic differences between adipoblasts derived from the epididymal adipose deposit of lean and obese 8‐week‐old Zucker and Wistar Diabetic Fatty (WDF) rats (15). In these strictly controlled culture conditions, obese‐derived adipoblasts expressed low levels of the late markers of differentiation (lipid accumulation, GPDH). In order to further characterize obese‐derived adipoblasts and analyze the critical relationship between growth and differentiation, growth arrest was induced in leanand obese‐derived cultures using sodium butyrate treatment. Addition of 2.5 mM sodium butyrate to the serum‐free medium from day 1 reduced markedly the growth of lean as well as obese‐derived cells. Adipoconversion of lean‐derived adipoblasts was not altered, similar levels of LPL and GPDH activities being obtained in control and butyrate‐treated groups. By contrast, a marked increase in both activities was observed in obese‐derived cultures, restoring the level of both markers of differentiation to the lean level. Similar results were obtained with adipoblasts derived from subcutaneous inguinal (ING) fat pad of obese Zucker as well as adipoblasts derived from ING and EPI fat deposits from obese WDF rats. Taken together, these results suggest that adipose deposits of these genetically obese rats contain a specific adipoblast population which differs from lean‐derived adipoblasts in respect to its adipoconversion capacity andlor its stage of commitment to differentiation.