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Variability in blood platelet inhibitory activity of Allium (Alliaceae) species accessions
Author(s) -
Goldman I. L.,
Schwartz B. S.,
Kopelberg M.
Publication year - 1995
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1995.tb15698.x
Subject(s) - allium , biology , cultivar , liliaceae , botany , genetic variability , horticulture , genotype , genetics , gene
In the past 20 yr, the presence of blood platelet inhibitory activity in plant species from the genus Allium has been confirmed by a range of clinical and in vitro investigations. Although a number of Allium species have been identified as possessing antiplatelet properties, little is known of the variability for this trait among accessions in these species. Experiments were conducted to assess variability in antiplatelet activity of 58 Allium (Alliaceae) accessions. Extracts were prepared from a diverse collection of 16 Allium species accessions, 33 cultivated accessions of Allium cepa including standard cultivars, inbred lines, and open‐pollinated populations, and nine Allium cepa plant introductions of diverse origin. Platelet inhibitory activity was determined via a platelet aggregation assay with human platelet‐rich plasma. Relative in vitro inhibition of platelet aggregation was measured for each accession and control samples, and inhibition constants (IC 50 ) were calculated. Dose‐dependent inhibition was observed and measured for each Allium accession. Significant ( P < 0.01) IC 50 variability was detected among accessions, with the lowest accession IC 50 values exhibiting nearly 50‐fold greater inhibition of aggregation than the highest accession IC 50 values. IC 5() variability among Allium cepa accessions was ≈ 12 times less than among Allium species accessions. Results from this investigation demonstrate substantial variability for efficacy of the antiplatelet factor among Allium accessions.