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DOES DAPI DETECT CYTOPLASMIC DNA?
Author(s) -
Johansen Bo,
Seberg Ole,
Petersen Gitte,
Arctander Peter
Publication year - 1995
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1995.tb12654.x
Subject(s) - plastid , dapi , biology , chloroplast dna , rubisco , pollen tube , dna , mitochondrial dna , chloroplast , pollen , biochemistry , microbiology and biotechnology , botany , gene , pollination , apoptosis
Ultrastructural observations have revealed that plastids are present in orchid pollen tubes, but the DNA‐binding fluorochrome 4',6‐diamidino‐2‐phenylindole (DAPI) does not localize any DNA in the pollen tube plastids at optimum binding and flourescence conditions. However, the plastids do contain DNA since the gene coding for the large subunit of rubisco (ribulose‐1,5‐bisphosphate carboxylase/oxygenase, rbc L) has been amplified by the polymerase chain reaction from orchid pollen tubes. It is therefore concluded that DAPI is an unreliable fluorochrome for detecting plastid DNA.