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Monoclonal antibodies against phloem P‐protein from plant tissue cultures. II. Taxonomic distribution of cross‐reactivity
Author(s) -
Tóth Karen F.,
Sjölund Richard D.
Publication year - 1994
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1994.tb15621.x
Subject(s) - biology , brassicaceae , phloem , monoclonal antibody , botany , sieve tube element , cucurbitaceae , arabidopsis , antibody , genetics , gene , mutant
P‐protein, a filamentous protein found in the sieve elements of most angiosperms, is believed to function in the sealing of phloem wound sites. We report here on the use of a highly sensitive immunomicroscopy assay to study the ability of P‐protein specific monoclonal antibodies RS21, RS22, and RS23, made against the P‐protein from Streptanthus tortuosus (Brassicaceae), to recognize the native P‐protein in a number of different plant genera. RS21, RS22, and RS23 all recognized the P‐protein in other genera within the Brassicaceae including Arabidopsis and in the closely related family, Capparaceae. RS21 and RS22 also were able to bind to the P‐protein in plants more distantly related to S. tortuosus . The labeling of P‐protein was also observed in the monocots Iris and Narcissus probed with RS21. No label was seen with members of the Poaceae that are reported to lack P‐protein. None of the monoclonal antibodies was able to bind to the P‐protein in members of the Cucurbitaceae.