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G enome origins of T riticum cylindricum , T riticum triunciale , and T riticum ventricosum (P oaceae) inferred from variation in restriction patterns of repeated nucleotide sequences: a methodological study
Author(s) -
Dubcovsky Jorge,
Dvorak Jan
Publication year - 1994
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1994.tb11454.x
Subject(s) - polyploid , ploidy , biology , phylogenetic tree , genetics , evolutionary biology , gene
Three methods of phylogenetic inferences on polyploid plants employing variation in restriction sites in repeated nucleotide sequences were compared. Allotetraploid Triticum species of well‐established origin were used as a model. Methods based on determination of the proportion of restriction fragments shared between a polyploid and its diploid relatives generated biased results because of uneven numbers of restriction fragments among diploid species and presence of common bands in phylogenetically related diploid species. A method employing restriction fragments unique to a diploid species (marker bands) was not affected by either factor and generated results consistent with cytogenetic inferences. It is shown that the latter method can be used to investigate the origin of a polyploid species even when one of its progenitors is extinct or when the polyploid and its diploid progenitors have diverged.